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KMID : 0359319930330020241
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Korean Journal of Veterinary Research
1993 Volume.33 No. 2 p.241 ~ p.247
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Immunocytochemical identification of Aujeszky´s disease viral antigens in nasal and circulating white blood cells from living pigs for early diagnosis
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김순복/Kim, Soon Bok
문운경/조희택/서정향/Moon, Oun Kyong/Cho, Hee Tack/Sur, Jung Hyang
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Abstract
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Forty day old piglets were intranasally inoculated with 2㎖ of Aujeszky´s disease virus (NYJ-1-87 stcain, 10^(7.0) TCID_(50)/0.2㎖), and the viral antigens were detected in nasal and circulating white blood cells for 20 days after inoculation by immunocytochemical method.
Antibody titers in the blood were also detected by neutralizing test and Aujeszky´s disease serodiagnostic kit(Choong Ang) in this periods.
l. Viral antigens were detected by the immunocytochemical technigue, and positive reactions were observated in nasal cells from the 2nd to the l0th days after inoculation and circulated white blood cells from the 4th to the 12th days after inoculation.
2. In neutralization test antibodies levels showed titers of 2 on the 8th day, 8 on the l5th day, 16 on the 18th day and 32 on the 20th day after inoculation. In serodiagnostic kit test positive reactions were observed after the 15th day after inoculation.
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KEYWORD
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